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We Beforehand identified PS1 mutants Lively inside the absence of NCT [29]. These mutants possessed two missense mutations in PS1: S438P at the ninth transmembrane domain along with one mutation dispersed through the entire molecule. These PS1 mutations Increased and modulated the γ-secretase activity and reduced the ratio of extended Aβs [32]. We then isolated Aph1 or Pen2 mutants capable of increase from the absence of NCT. right after we experienced screened cells with Pen2 or Aph1 mutations along with the PS1 S438P mutation, we located 13 Aph1 mutants that grew in the absence of NCT (desk one and Figure one).
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